[6]-Gingerol (Synonyms: (S)-(+)-[6]Gingerol)

Name: [6]-Gingerol
Brand: MedChemExpress
SKU: HY-14615
Rating: 5.0 (5 reviews)

Cat. No.: HY-14615 Purity: 99.44%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

[6]-Gingerol is an active compound isolated from Ginger (Zingiber officinale), exhibits a variety of biological activities including anticancer, anti-inflammation, and anti-oxidation.

For research use only. We do not sell to patients.

[6]-Gingerol Chemical Structure

CAS No. : 23513-14-6

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 73	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	USD 73	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 66	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 198	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 319	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 572	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of [6]-Gingerol:

[6]-Gingerol (Standard) Get quote

5 Publications Citing Use of MCE [6]-Gingerol

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NUAK1 NUAK2 AMPK AMPK α1β1γ1 AMPK α2β1γ1 AMPK α1β2γ1 AMPK α2β2γ1

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

[6]-Gingerol is an active compound isolated from Ginger (Zingiber officinale), exhibits a variety of biological activities including anticancer, anti-inflammation, and anti-oxidation.

IC₅₀ & Target^[2]

AMPK

Apoptosis

Cellular Effect

Cell Line	Type	Value	Description	References
DU-145	IC₅₀	95 mg/mL Compound: Gingerol	Antiproliferative activity against human DU145 cells after 72 hrs by Alamar blue assay Antiproliferative activity against human DU145 cells after 72 hrs by Alamar blue assay	[PMID: 31336310]
LNCaP	IC₅₀	75 mg/mL Compound: Gingerol	Antiproliferative activity against human LNCAP cells after 72 hrs by Alamar blue assay Antiproliferative activity against human LNCAP cells after 72 hrs by Alamar blue assay	[PMID: 31336310]
LNCaP C4-2	IC₅₀	512 mg/mL Compound: Gingerol	Antiproliferative activity against human C4-2 cells after 72 hrs by Alamar blue assay Antiproliferative activity against human C4-2 cells after 72 hrs by Alamar blue assay	[PMID: 31336310]
LNCaP C4-2B	IC₅₀	240 mg/mL Compound: Gingerol	Antiproliferative activity against human C4-2B cells after 72 hrs by Alamar blue assay Antiproliferative activity against human C4-2B cells after 72 hrs by Alamar blue assay	[PMID: 31336310]
NCI-H1299	IC₅₀	150 μM Compound: [6]-gingerol	Cytotoxicity against human H1299 cells assessed as growth inhibition after 24 hrs by MTT assay Cytotoxicity against human H1299 cells assessed as growth inhibition after 24 hrs by MTT assay	[PMID: 22246389]
PC-3	IC₅₀	250 mg/mL Compound: Gingerol	Antiproliferative activity against human PC3 cells after 72 hrs by Alamar blue assay Antiproliferative activity against human PC3 cells after 72 hrs by Alamar blue assay	[PMID: 31336310]
RBL-2H3	IC₅₀	185.1 μM Compound: 5	Cytotoxicity against rat RBL2H3 cells after 12 hrs by MTT assay Cytotoxicity against rat RBL2H3 cells after 12 hrs by MTT assay	[PMID: 19271742]

In Vitro

[6]-gingerol inhibits colon cancer cell proliferation and induced apoptosis, while the normal colon cells are unaffected. [6]-gingerol down-regulates phorbol myristate acetate induced phosphorylation of ERK1/2 and JNK MAP kinases and activation of AP-1 transcription factor, but has only little effects on phosphorylation of p38 MAP kinase and activation of NF-kappa B^[1]. [6]-gingerol treatment is shown to restore impaired intestinal barrier function and to suppress proinflammatory responses in DSS-treated Caco-2 monolayers. AMPK is activated on [6]-gingerol treatment^[2]. Treatment with [6]-gingerol results in a significant decrease in the viability of osteosarcoma cells in a dose-dependent fashion. In parallel, the number of cells arrested at the sub-G1 cell cycle phase is significantly increased. [6]-gingerol induces activation of caspase cascades and regulates cellular levels of Bcl2 and Bax^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

In animal studies, [6]-gingerol significantly ameliorates DSS-induced colitis by restoration of body weight loss, reduction in intestinal bleeding, and prevention of colon length shortening. In addition, [6]-gingerol suppresses DSS-elevated production of proinflammatory cytokines (IL-1β, TNFα, and IL-12)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

294.39

Formula

C₁₇H₂₆O₄

CAS No.

23513-14-6

Appearance

Solid

Color

White to off-white

SMILES

CCCCC[C@H](O)CC(CCC1=CC=C(O)C(OC)=C1)=O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (169.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3969 mL	16.9843 mL	33.9685 mL
5 mM	0.6794 mL	3.3969 mL	6.7937 mL
10 mM	0.3397 mL	1.6984 mL	3.3969 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (8.49 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 25 mg/mL (84.92 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.45%

Select Batch:

Data Sheet (275 KB) SDS (480 KB)

COA (243 KB) HNMR (262 KB) LCMS (265 KB)

Handling Instructions (2659 KB)

References

[1]. Radhakrishnan EK, et al. [6]-Gingerol induces caspase-dependent apoptosis and prevents PMA-induced proliferation in colon cancer cells by inhibiting MAPK/AP-1 signaling. PLoS One. 2014 Aug 26;9(8):e104401. [Content Brief]

[2]. Chang KW, et al. 6-Gingerol modulates proinflammatory responses in dextran sodium sulfate (DSS)-treated Caco-2 cells and experimental colitis in mice through adenosine monophosphate-activated protein kinase (AMPK) activation. Food Funct. 2015 Oct;6(10):3334-41. [Content Brief]

[3]. Fan J, et al. 6-Gingerol inhibits osteosarcoma cell proliferation through apoptosis and AMPK activation. Tumour Biol. 2015 Feb;36(2):1135-41. [Content Brief]

Cell Assay ^[1]	[6]-gingerol stock (20 mg/mL) is prepared in ethanol and the working concentrations are prepared by diluting this stock in dimethyl sufoxide (DMSO). For MTT assay, 5×10³ cells/well of human colon cancer cells and 10⁴ cells/well of mouse IECs are seeded in 96-well plates. Cells are treated with [6]-gingerol for 48 h,72 h or 96 h before performing MTT assay and for 16 h before Annexin-V staining^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[2]	Mice: Mice with DSS-induced colitis are given different oral dosages of [6]-gingerol daily for 14 days. Body weight and colon inflammation are evaluated, and level of proinflammatory cytokines in colon tissues is measured^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Radhakrishnan EK, et al. [6]-Gingerol induces caspase-dependent apoptosis and prevents PMA-induced proliferation in colon cancer cells by inhibiting MAPK/AP-1 signaling. PLoS One. 2014 Aug 26;9(8):e104401. [Content Brief] [2]. Chang KW, et al. 6-Gingerol modulates proinflammatory responses in dextran sodium sulfate (DSS)-treated Caco-2 cells and experimental colitis in mice through adenosine monophosphate-activated protein kinase (AMPK) activation. Food Funct. 2015 Oct;6(10):3334-41. [Content Brief] [3]. Fan J, et al. 6-Gingerol inhibits osteosarcoma cell proliferation through apoptosis and AMPK activation. Tumour Biol. 2015 Feb;36(2):1135-41. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.3969 mL	16.9843 mL	33.9685 mL	84.9214 mL
	5 mM	0.6794 mL	3.3969 mL	6.7937 mL	16.9843 mL
	10 mM	0.3397 mL	1.6984 mL	3.3969 mL	8.4921 mL
	15 mM	0.2265 mL	1.1323 mL	2.2646 mL	5.6614 mL
	20 mM	0.1698 mL	0.8492 mL	1.6984 mL	4.2461 mL
	25 mM	0.1359 mL	0.6794 mL	1.3587 mL	3.3969 mL
	30 mM	0.1132 mL	0.5661 mL	1.1323 mL	2.8307 mL
	40 mM	0.0849 mL	0.4246 mL	0.8492 mL	2.1230 mL
	50 mM	0.0679 mL	0.3397 mL	0.6794 mL	1.6984 mL
	60 mM	0.0566 mL	0.2831 mL	0.5661 mL	1.4154 mL
	80 mM	0.0425 mL	0.2123 mL	0.4246 mL	1.0615 mL
	100 mM	0.0340 mL	0.1698 mL	0.3397 mL	0.8492 mL

[6]-Gingerol Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

[6]-Gingerol23513-14-6(S)-(+)-[6]GingerolAMPKApoptosisAMP-activated protein kinaseInhibitorinhibitorinhibit

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[6]-Gingerol (Synonyms: (S)-(+)-[6]Gingerol)

Customer Review

Other Forms of [6]-Gingerol:

[6]-Gingerol Related Antibodies

5 Publications Citing Use of MCE [6]-Gingerol

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All AMPK Isoform Specific Products:

Biological Activity

Protocol

Purity & Documentation

References

Customer Review

[6]-Gingerol Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

[6]-Gingerol Related Classifications

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